Identification of the Microbial Population Found in Water Sources in and around San Salvador Island, Bahamas
DOI:
https://doi.org/10.15362/ijbs.v20i1.196Keywords:
Microbiology, San Salvador, Bahamas, Marine biology - BahamasAbstract
San Salvador Island in The Bahamas is home to approximately 1,200 people, and a popular vacation destination. In order to expand our knowledge of the bacterial population found on and around the island, and to assess possible health risks, we analyzed and identified the cultivable bacterial population found in several lakes and ponds throughout the island. The sites tested were located on the northern, north-eastern, eastern, and western districts, as well as one lake located inland. Ten sites with varying salinity, levels of oxygen, visibility, and distance from the ocean were analyzed. The nature of the bacteria present in these sites was identified by microscopy, as well as a series of biochemical tests based on bacterial metabolism. Seven bacterial species, predominantly from the genera Staphylococcus and Klebsiella were identified. Most bacteria identified are part of the normal microbiota of the skin and the gastro-intestinal tract of human and mammals, and should not be considered a danger for the health of the majority of the population and tourists of the island. We also isolated bacteria capable of fixing atmospheric nitrogen, a hallmark of marine bacterial populations. Overall, this study enabled us to add to the repertoire of bacterial species isolated and identified in the diverse marine environments found on San Salvador Island.References
Baldursson, S., & Karanis, P. (2011). Waterborne transmission of protozoan parasites: Review of worldwide outbreaks: an update, 2004-2010. Water Research, 45(20), 6603-6614. https://doi.org/10.1016/j.watres.2011.10.013
Balows, A., Truper, H. G., Dworkin, M., Harder, W., & Schleifer, K.-H. (Eds.). (1991). The genus Staphylococcus. In The Prokaryotes: a Handbook on the biology of bacteria: Ecophysiology, isolation, identification, application (2nd. ed., pp. 1369-1420). Springer.
Bannerman, T. L., & Peacock, S. J. (2007). Staphylococcus, Micrococcus, and other catalase-positive cocci. In P. R. Murray, E. J. Baron, J. H. Jorgensen, M. L. Landry & M. A. Pfaller (Eds.), Manual of clinical microbiology (9th ed., pp. 390-404). ASM Press.
Bauman, R. W. (2014). Microbiology with diseases by taxonomy (4th ed.). Pearson Education.
Cabral, J. P. S. (2010). Water microbiology, bacterial pathogens and water. International Journal of Environmental Research and Public Health, 7(10), 3657-3703. 10.3390/ijerph7103657
Emery, W. J., & Thomson, R. E. (2004). Data analysis methods in physical oceanography. 2nd rev. ed. Elsevier.
Funke, G., von Graevenitz, A., Clarridge, J. E, & Bernard, K. A. (1997). Clinical microbiology of coryneform bacteria. Clinical Microbiology Reviews, 10(1), 125-159. https://doi.org/10.1128/CMR.10.1.125
Garrity, G. M., Brenner, D. J., Krieg, N. R., & Staley, J. T., (Eds.). (2009). Bergey's manual of systemic bacteriology, volume 2. (2nd ed.) Springer.
Gerace, D. T., Ostrander, G. K., & Smith, G. W. (1998). San Salvador, Bahamas. In B. Kjerfve (Ed.), CARICOMP Caribbean coral reef, seagrass and mangrove sites (pp. 229-246). Paris: UNESCO. http://www.unesco.org/csi/pub/papers/papers3.pdf
Godfree, A. F., Kay, D., & Wyer, M. D. (1997). Faecal streptococci as indicators of faecal contamination in water. Journal of Applied Microbiology, 83(S1), 110-119. https://doi.org/10.1046/j.1365-2672.83.s1.12x
Hecker, M., & Völker, U. (2001). General stress response of Bacillus subtilis and other bacteria. Advances in microbial physiology, 44, 35-91. https://doi.org/10.1016/S0065-2911(01)44011-2
Hill, S., Turner, G. L., & Bergersen, F. J. (1984). Synthesis and activity of nitrogenase in Klebsiella pneumoniae exposed to low concentrations of oxygen. Journal of general microbiology, 130(5), 1061-1067. https://doi.org/10.1099/00221287-130-5-1061
Holt, J. G. (2000). Bergey’s manual of determinative bacteriology. (9th ed.). Lippincott, Williams & Wilkins.
La Rosa, G., Fratini, M., della Libera, S., Iaconelli, M., & Muscillo, M. (2012). Emerging and potentially emerging viruses in water environments. Annali dell'Istituto superiore di sanità, 48(4), 397-406. https://doi.org/10.4415/ANN_12_04_07
Lipsky, B. A., Goldberger, A. C., Tompkins, L. S., & Plorde, J. J. (1982). Infections caused by nondiphtheria corynebacteria. Review of Infectious Diseases, 4(6), 1220-1235. https://doi.org/10.1093/clinids/4.6.1220
Mahaffey, C., Michaels, A. F., & Capone, D. G. (2005). The conundrum of marine N2 fixation. American Journal of Science, 305(6-8), 546-595. https://doi.org/10.2475/ajs.305.6-8.546
Mylroie, J. R., & Thoren, L. A. (2007). Variations in coliform bacterial counts on San Salvador, Bahamas {Conference Proceedings]. 11th Symposium on the Natural History of The Bahamas. San Salvador, Bahamas: Gerace Research Center. http://www.geraceresearchcentre.com/pdfs/11thNatHist/21_JRMylroieThoren_11thNatHist.pdf
Nester, E. W., Anderson, D. G., Evans Roberts Jr., C., & Nester, M. T. (2012). Microbiology, a human perspective (7th ed.). McGraw Hill.
Paerl, H. W., Steppe, T. F., Buchan, K. C., & Potts, M. (2003). Hypersaline cyanobacterial mats as indicators of elevated tropical hurricane activity and associated climate change. AMBIO: A Journal of the Human Environment, 32(2), 87-90. https://doi.org/10.1579/0044-7447-32.2.87
Palacios, L., Vela, A. I., Molin, K., Fernández, A., Latre, M. V., Chacón, G., ... & Fernández-Garayzábal, J. F. (2010). Characterization of some bacterial strains isolated from animal clinical materials and identified as Corynebacterium xerosis by molecular biological techniques. Journal of clinical microbiology, 48(9), 3138-3145. https://doi.org/10.1128/JCM.02373-09
Park, L. E. (2012). Comparing two long-term hurricane frequency and intensity records from San Salvador Island, Bahamas. Journal of Coastal Research, 28(4), 891-902. https://doi.org/10.2112/JCOASTRES-D-11-00065.1
Shaklee, R. (1994). In Columbus’s footsteps: Geography of San Salvador Island, the Bahamas. Bahamian Field Station.
San Salvador, Bahamas: San Salvador’s history. (2013). http://www.the-bahamas.net/the-islands/san-salvador-bahamas/.
United States Environmental Protection Agency. (2012). Water contaminant candidate list. http://water.epa.gov/scitech/drinkingwater/dws/ccl/ccl3.cfm).
Vos, P., Garrity, G., Jones, D., Krieg, N. R., Ludwig, W., Rainey, F. A., Whitman, W. B. (Eds.). (2009). Bergey's manual of systematic bacteriology, volume 3. (2nd ed.). Springer.
Whitman, W. B., Goodfellow, M., Kämpfer, P., Busse, H.-J., Trujillo, M. E., Suzuki, K.-I., & Ludwig, W. (Eds.). (2012). Bergey's manual of systematic bacteriology, volume 5. (2nd ed.). Springer.
World Health Organization. (2014). Global Health Observatory Data Repository. http://apps.who.int/gho/data/node.main.
Yannarell, A. C., Steppe, T. F., & Paerl, H. W. (2006). Genetic variance in the composition of two functional groups (diazotrophs and cyanobacteria) from a hypersaline microbial mat. Applied and environmental microbiology, 72(2), 1207-1217. https://doi.org/10.1128/AEM.72.2.1207-1217.2006
Zehr, J. P., Waterbury, J. B., Turner, P. J., Montoya, J. P., Omoregie, E., Steward, G. F., ... & Karl, D. M. (2001). Unicellular cyanobacteria fix N2 in the subtropical North Pacific Ocean. Nature, 412(6847), 635-638. https://doi.org/10.1038/35088063
Zehr, J. P. (2011). Nitrogen fixation by marine cyanobacteria. Trends in microbiology, 19(4), 162-173. https://doi.org/10.1016/j.tim.2010.12.004